CheckMate(TM) Mammalian TwoHybrid System ยี่ห้อ Promega (Stroge. Temp -30C TO -10C)
รหัสสินค้า : E2440
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CheckMate™ Mammalian Two-Hybrid System
Powerful System for Detecting Protein:Protein Interactions In Vivo
- Mammalian system for studying interactions in cell line of choice
- Study post-translational modifications including glycosylation, phosphorylation and acylation
Two-hybrid systems are extremely powerful methods for detecting protein:protein interactions in vivo. The basis of two-hybrid systems is the modular domains found in some transcription factors: a DNA-binding domain, which binds to a specific DNA sequence, and a transcriptional activation domain, which interacts with the basal transcriptional machinery. A transcriptional activation domain in association with a DNA-binding domain will promote the assembly of RNA polymerase II complexes at the TATA box and increase transcription.
In the CheckMate™ Mammalian Two-Hybrid System the DNA-binding domain and the transcriptional activation domain, produced by separate plasmids, are closely associated when one protein ("X") fused to a DNA-binding domain interacts with a second protein ("Y") fused to a transcriptional activation domain. In this system, interaction between proteins X and Y results in transcription of a reporter gene.
For more information, see the Protocols & Applications Guide.
Application
- Confirming suspected interactions between two proteins and identifying domains/residues involved in protein:protein interactions.
Reference
- Sadowski, I. et al. (1988) Nature 335, 563–4.
ItemPart #SizeAvailable Separately
pBIND Vector
E245A1 × 20μgView Product
pACT Vector
E246A1 × 20μgView Product
pBIND-Id Control Vector
E247A1 × 20μgView Product
pACT-MyoD Control Vector
E248A1 × 20μgView Product
pG5luc VectorE249A2 × 20μg
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