pGL4.75[hRluc/CMV] Vector, 20ug ยี่ห้อ Promega (Stroge. Temp -30C TO -10C)
รหัสสินค้า : E6931
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Promoter-Driven Control Renilla Luciferase Vectors
Vectors Co-transfected in Dual-Reporter Assays as the Control Vector
- Co-transfect into cells where firefly luciferase is the experimental reporter gene
- CMV, SV40 and TK promoter options offer different expression levels suitable for most dual-reporter assays
The pGL4.73[hRluc/SV40], pGL4.74[hRluc/TK] and pGL4.75[hRluc/CMV] Vectors are promoter-driven Renilla control vectors commonly co-transfected with experimental firefly luciferase vectors for use in dual-reporter assays such as the Dual-Luciferase® or Dual-Glo® Reporter Assay Systems. The control Renilla vectors should give an almost invariant level of activity, while the experimental firefly vector varies with treatment.
Applications
- Transcription regulation.
- Virus-cell interactions.
- Compound screening.
- Post-translational modifications.
- GPCR signaling.
- Cell signaling.
- Promoter analysis.
About the pGL4 Reporter Vectors
The pGL4 Vectors offer increased reporter gene expression with codon optimization of synthetic genes for mammalian expression and reduced background and risk of expression artifacts with removal of cryptic DNA regulatory elements and transcription factor binding sites. Renilla luciferase has options that offer improved temporal response with the destabilized Rapid Response™ luciferase genes.
Sequence Information
pGL4.73[hRluc/SV40] Vector GenBank® Accession Number AY738229 and vector sequence text file.
pGL4.74[hRluc/TK] Vector GenBank® Accession Number AY738230 and vector sequence text file.
pGL4.75[hRluc/CMV] Vector GenBank® Accession Number AY738231 and vector
ItemPart #Size
pGL4.75[hRluc/CMV] Vector
E693A1 × 20μg
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