pGL4.83(hRlucP/Puro)Vector, 20ug ยี่ห้อ Promega (Stroge. Temp -30C TO -10C)
รหัสสินค้า : E7511
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Promoterless Renilla Luciferase Vectors with Puromycin Selection
Cloning Vectors For Use as Control Reporter Vectors Offering Different Reporter Protein Stabilities
- Designed for cloning a putative promoter element for investigating gene transcription control regions
- Available with three varieties of engineered Renilla luciferase genes: hRluc, hRlucP or hRlucCP
- Select for stably transfected cells using puromycin
The pGL4.82[hRluc/Puro], pGL4.83[hRlucP/Puro] and pGL4.84[hRlucCP/Puro] Vectors are designed primarily to accept a putative promoter element for investigation of important regions controlling gene transcription. Alternatively, these vectors may be used as promoterless control vectors in a dual-reporter system with a firefly luciferase vector serving as the experimental vector. The promoterless vectors are available with three varieties of engineered Renilla luciferase genes: hRluc, hRlucP or hRlucCP, and offer a selectable marker, puromycin. The inserts cloned into these vectors can easily be transferred using the multiple cloning site and a unique SfiI transfer scheme.
Applications
- Transcription regulation.
- Virus-cell interactions.
- Compound screening.
- Post-translational modifications.
- Promoter analysis.
How the Engineered Renilla Luciferase Genes Work
The hRluc gene is engineered to remove most cryptic transcription factor binding sites and improve mammalian expression through codon optimization.
About the pGL4 Reporter Vectors
The pGL4 Vectors offer increased reporter gene expression with codon optimization of synthetic genes for mammalian expression and reduced background and risk of expression artifacts with removal of cryptic DNA regulatory elements and transcription factor binding sites. Renilla luciferase has options that offer improved temporal response with the destabilized Rapid Response™ luciferase genes.
ItemPart #Size
pGL4.83[hRlucP/Puro] Vector
E751A1 × 20μg
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