Human FcRn / FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (SPR & BLI & HPLC-verified)

Synonym

• FcRn, FCGRT & B2M

Source

• Human FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag(FCM-H5286) is expressed from human 293 cells (HEK293). It contains AA Ala 24 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M) (Accession # P55899-1 (FCGRT) & P61769-1 (B2M)).
• Predicted N-terminus: Ala 24 (FCGRT) & Ile 21 (B2M)

Molecular Characterization

• Human FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag, produced by co-expression of FCGRT and B2M, has a calculated MW of 31.2 kDa (FCGRT) and 13.1 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus and subunit Beta-2 microglobulin (B2M) is fused with Strep II-tag at the C-terminus. The reducing (R) protein migrates as 33 kDa (FCGRT) and 13 kDa (B2M) respectively due to glycosylation.

Endotoxin

• Less than 1.0 EU per μg by the LAL method.

Purity

• >95% as determined by SDS-PAGE.
• >90% as determined by SEC-HPLC.

Formulation

• Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Contact us for customized product form or formulation.

Reconstitution

• Please see Certificate of Analysis for specific instructions.
• For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

• For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Please avoid repeated freeze-thaw cycles.
• This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 12 months under sterile conditions after reconstitution.

SDS-PAGE

Human FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-SPR

Immobilized Human FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (Cat. No. FCM-H5286) on CM5 Chip via Anti-His antibody, can bind Herceptin with an affinity constant of 0.261 μM as determined in a SPR assay (Biacore T200) (QC tested).

Immobilized Human FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (Cat. No. FCM-H5286) on CM5 Chip, can bind Herceptin with an affinity constant of 1.19 μM as determined in a SPR assay (Biacore T200) (Routinely tested).

Bioactivity-BLI

Loaded Human FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (Cat. No. FCM-H5286) on SA Biosensor via Biotin his antibody, can bind Herceptin with an affinity constant of 0.27 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

SEC-HPLC

The purity of Human FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (Cat. No. FCM-H5286) was greater than 90% as determined by SEC-HPLC.

Herceptin immobilized on CM4 Chip can bind Human FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (Cat. No. FCM-H5286) with an affinity constant of 1.4 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).

Loaded Human FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (Cat. No. FCM-H5286) on AR2G Biosensor, can bind Herceptin with an affinity constant of 0.35 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).