EZBlue™ Gel Staining Reagent ยี่ห้อ Sigma

PROPERTIES

technique(s)

protein staining: suitable

Quality Level

200

storage temp.

2-8°C

DESCRIPTION

General description

Convenient, sensitive, and safe, EZBlue^™ Coomassie Brilliant Blue G-250 colloidal protein stain improves protein electrophoresis results while significantly reducing staining time. Conveniently packaged, EZBlue requires no messy weigh-ups or additions of methanol or acid. As a colloidal stain, it reacts only with proteins, not the gel itself. Background staining is reduced, so protein bands can be visualized almost immediately. No destaining step is required, although a water wash may intensify bands and clarify the background. Most impressively, EZBlue is extremely sensitive, detecting as little as 5 ng of protein.

Application

EZBlue^® Gel Staining Reagent has been used as a staining reagent in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).[1][2]

Features and Benefits

·       Premixed solution eliminates the time and effort required to prepare the stain

·       Increased sensitivity ensures that low abundance proteins can be detected (as little as 5 ng)

·       Rapid reaction significantly reduces the amount of time required to stain and rinse

·       No solvent waste so you save time and money by eliminating hazardous material disposal

Other Notes

A ready-to-use Brilliant Blue G-250 based protein stain for "one step" ultrasensitive detection on polyacrylamide gels and PVDF membranes.

Legal Information

EZBlue is a trademark of Sigma-Aldrich Co. LLC

Sigma-Aldrich is a registered trademark of Sigma-Aldrich Co. LLC