pFC16K HaloTag® CMVd2 Flexi® Vector, 20ug ยี่ห้อ Promega (Stroge. Temp -30C TO -10C)
รหัสสินค้า : G1571
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pFC16A and pFC16K HaloTag® CMVd2 Flexi® Vectors
We are sorry, but these products are discontinued.
Constitutive Protein Expression in Mammalian Cells
The pFC16A and pFC16K HaloTag® CMVd2 Flexi® Vectors are configured to append the HaloTag® to the carboxyterminus of the protein fusion partner and provide constitutive protein expression in mammalian cells using a modified human cytomegalovirus (CMV) immediate early enhancer/promoter, CMVd2. The vectors can be used for both stable and transient gene expression; for stable expression, cotransfection with a vector containing a selectable marker is required. A choice of ampicillin (A) or kanamycin (K) is provided with these vectors.
Note: Flexi® Vectors contain the lethal barnase gene to reduce background colonies without inserts during the subcloning procedure. Using the Flexi® Vector Cloning System replaces the barnase gene with your insert. These vectors, as purchased, cannot be cultured in normal laboratory strains of E. coli without an insert.
Application
- Clone ORF and conveniently transfer these inserts to a variety of vectors designed for multiple applications.
GenBank® Accession Numbers and Sequence Information
pFC16A HaloTag® CMVd2 GenBank® Accession Number EU332339; pFC16A HaloTag® CMVd2 Vector sequence text file.
pFC16K HaloTag® CMVd2 GenBank® Accession Number EU332340; pFC16K HaloTag® CMVd2 Vector sequence text file.
ItemPart #Size
pFC16K HaloTag® CMVd2 Flexi® Vector
G157A1 × 20μg
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