pGEM® DNA Markers

Fifteen DNA Fragments of 36–2,645bp, Combined From Three Separate Digests of pGEM®-3 Vector

• Load 1μl/lane
• Supplied at a concentration of 1µg/μl
• Supplied with 6X Blue/Orange Loading Dye
• Now ships at ambient temperature

Conventional DNA Digest Markers are created by digesting DNA (i.e., λ DNA, ΦX174 replicative form DNA or plasmids) to completion with one or more restriction enzymes. The enzymes are heat-inactivated, and the DNA fragments are either phenol-extracted, then ethanol-precipitated or just ethanol-precipitated. The precipitated fragments are resuspended in storage buffer. These markers are not intended for quantitative analysis.

The pGEM® DNA Markers have 15 phenol-extracted, ethanol-precipitated DNA fragments ranging in size from 36bp to 2,645bp. These unique markers are generated from separate digests of pGEM®-3 Vector DNA with HinfI, RsaI and AvaII later combined to form the markers.

Storage Buffer: 10mM Tris-HCl (pH 7.8), 1mM EDTA.

ItemPart #Size

pGEM® DNA Markers

G174A1 × 50μg

Blue/Orange 6X Loading Dye

G190A1 × 1ml

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