pFN22A and pFN22K HaloTag® CMVd1 Flexi® Vectors

Constitutive Protein Expression in Mammalian Cells

The pFN22A and pFN22K HaloTag® CMVd1 Flexi® Vectors are configured to append the HaloTag® to the aminoterminus of the protein fusion partner and provide constitutive protein expression in mammalian cells using a modified human cytomegalovirus (CMV) immediate-early enhancer/promoter, CMVd1. The vector can be used for both stable and transient gene expression; for stable expression, cotransfection with a vector containing a selectable marker is required. These vectors are provided with a choice of ampicillin (A) or kanamycin (K) antibiotic resistance genes.

Note: Flexi® Vectors contain the lethal barnase gene to reduce background colonies without inserts during the subcloning procedure. Using the Flexi® Vector Cloning System replaces the barnase gene with your insert. These vectors, as purchased, cannot be cultured in normal laboratory strains of E. coli without an insert.

Application

• Clone ORF and conveniently transfer these inserts to a variety of vectors designed for multiple applications.

GenBank® Accession Numbers and Sequence Information

pFN22A HaloTag® CMVd1 GenBank® Accession Number EU621376; pFN22A HaloTag® CMVd1 Vector sequence text file.

pFN22K HaloTag® CMVd1 GenBank® Accession Number EU621377; pFN22K HaloTag® CMVd1 Vector sequence text file.

ItemPart #Size

pFN22K HaloTag® CMVd1 Flexi® Vector

G285A1 × 20μg

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