DeadEnd™ Fluorometric TUNEL System

A Classic TUNEL Assay to Detect and Quantitate Apoptotic Cells

• Save time and money
• Detect apoptosis in cultured cells or FFPE tissue sections

The DeadEnd™ Fluorometric TUNEL System is a classic TUNEL Assay designed for the specific detection and quantitation of apoptotic cells within a cell population. This system measures nuclear DNA fragmentation, an important biochemical hallmark of apoptosis in many cell types, providing simple, accurate and rapid detection of apoptotic cells in situ at the single-cell level or in cell suspensions. The DeadEnd™ Fluorometric TUNEL System measures the fragmented DNA of apoptotic cells by catalytically incorporating fluorescein-12-dUTP at 3´-OH DNA ends using Terminal Deoxynucleotidyl Transferase (TdT), which forms a polymeric tail using the principle of the TUNEL (TdT-mediated dUTP Nick-End Labeling) assay. The fluorescein-12-dUTP-labeled DNA can then be visualized directly by fluorescence microscopy or quantitated by flow cytometry.

 Features and Benefits

• System provides sufficient reagents for 60 assays of 50μl each and saves money.
• Direct incorporation of fluorescent nucleotide reduces number of incubation steps.
• Use to detect apoptosis in cultured cells and formalin-fixed, paraffin-embedded tissue sections.
• Plastic coverslips provided simplify sample handling.

Note: The protocol for the DeadEnd™ TUNEL Assay recommends an optional DNase I treatment of samples as a positive control to detect DNA fragmentation. RQ1 RNase-Free DNase (Cat.# M6101) can be used to generate the positive control and is available separately.

ItemPart #SizeAvailable Separately

Plastic Coverslips

G326A1 × 60 pack

Equilibration Buffer

G327C1 × 9.6ml

Nucleotide Mix

G328A6 × 50μl

SSC, 20X

G329A1 × 70ml

Terminal Deoxynucleotidyl Transferase (recombinant)

M828B3 × 600u

Proteinase K

V302A1 × 10mgView Product

แหล่งที่มา : https://worldwide.promega.com/products/cell-health-assays/apoptosis-assays/deadend-fluorometric-tunel-system/?catNum=G3250