HDAC-Glo(TM) I/II Assay, 100ml ยี่ห้อ Promega (Stroge. Temp -30C TO -10C)
รหัสสินค้า : G6422
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HDAC-Glo™ I/II Assays and Screening System
Detects HDAC Class I and II Enzyme Activity
- Simple add-mix-measure protocol
- Use purified enzymes, extracts or cells directly in culture plates
- Screening System includes HeLa Nuclear Extract
Predictive, Flexible Assays for HDAC Activity
The HDAC-Glo™ I/II Assays and Screening Systems are single-reagent-addition, homogeneous, luminescent assays that measure the relative activity of histone deacetylase (HDAC) class I and II enzymes using purified enzymes, extracts or cells directly in culture plates. The add-mix-measure protocol allows for simple measurement of deacetylating activities and easy implementation from benchtop to screening. The HDAC-Glo™ I/II Assays and Screening Systems can be used to determine HDAC inhibitor potency, selectively profile HDAC inhibitors, correlate HDAC inhibitor potency with cellular fate in same-well multiplexed viability assays or determine off-target HDAC effects of compounds.
The assays use an acetylated, live-cell-permeant, luminogenic peptide substrate that can be deacetylated by HDAC activities. Deacetylation of the peptide aminoluciferin substrate is measured using a coupled enzymatic system in which a protease in the Developer Reagent cleaves the deacetylated peptide from the aminoluciferin substrate, releasing aminoluciferin, which is quantified in a reaction using Ultra-Glo™ recombinant firefly luciferase.
The assay reaction is typically complete within 15–45 minutes with no sample manipulation. The HDAC-mediated luminescent signal is persistent, with a half-life of greater than 3 hours, making the protocol amenable to automation in high-throughput formats and compatible with luminometers without injectors and allowing batch processing of multiwell plates. The HDAC assay is broadly useful for class I and II enzymes. The HDAC-Glo™ I/II Assays are highly sensitive, allowing you to obtain a dynamic range 10- to 100-fold higher than comparable fluorescence methods.
ItemPart #Size
HDAC-Glo™ I/II Buffer
G648B2 × 125ml
HDAC-Glo™ I/II Substrate
G649B1 × 1 vial
Developer Reagent
G653B2 × 50μl
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