L-Glutamic acid from non-animal source, meets EP testing specifications, suitable for cell culture, 98.5–100.5% ยี่ห้อ Sigma

PROPERTIES

biological source

non-animal source

Quality Level

200

Agency

meets EP testing specifications

Assay

98.5-100.5%

form

powder

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

color

white

mp

205 °C (dec.) (lit.)

solubility

1 M HCl: 100 mg/mL

density

1.54 g/cm3 at 20 °C

anion traces

chloride (Cl-): ≤200 ppm

sulfate (SO42-): ≤200 ppm

cation traces

As: ≤1 ppm, passes test

Fe: ≤10 ppm, passes test

NH4+: ≤200 ppm, passes test

application(s)

pharmaceutical (small molecule)

SMILES string

N[C@@H](CCC(O)=O)C(O)=O

InChI

1S/C5H9NO4/c6-3(5(9)10)1-2-4(7)8/h3H,1-2,6H2,(H,7,8)(H,9,10)/t3-/m0/s1

InChI key

WHUUTDBJXJRKMK-VKHMYHEASA-N

Gene Information

human ... CCR2(1231) , GRIA1(2890) , GRIA2(2891) , GRIA4(2893) , GRIK1(2897) , GRIK2(2898) , GRIK3(2899) , GRIK5(2901) , GRIN2B(2904) , GRM2(2912) , SLC1A1(6505) , SLC1A2(6506)

rat ... Gria1(50592) , Grik1(29559) , Grik2(54257) , Grik4(24406) , Grin2a(24409) , Grm1(24414) , Grm2(24415) , Grm3(24416) , Grm4(24417) , Grm5(24418) , Grm6(24419) , Grm7(81672) , Slc1a2(29482)

DESCRIPTION

Application

L-Glutamic acid has been used as a supplement in for cell culture media to culture hippocampal neurons and hT-PA (human tissue plasminogen activation) producing cell line.[1][2][3]

Biochem/physiol Actions

Glutamic acid, or glutamate, the salt form of glutamic acid, functions as a neurotransmitter and also serves as a precursor to other neurotransmitters such as γ-aminobutyric acid.[4] Glutamic acid also plays a key role in many metabolic pathways, that controls growth, reproduction, maintenance and immunity.[5] It is converted to α-ketoglutarate, a key component of the TCA (tricarboxylic acid) cycle, and a precursor for the biosynthesis of nucleic acids and certain amino acids.[5][6] In cells, glutamine is converted to glutamate by the enzyme glutaminase.[6] Glutamine serves as a source of energy for rapidly dividing cells comprising lymphocytes, enterocytes, macrophages and tumors. Glutamine mediates protein turnover via cellular mTOR (mammalian target of rapamycin) signaling. It is also known to be associated with the inhibition of apoptosis.[5]

An excitatory amino acid neurotransmitter that is an agonist at all subtypes of glutamate receptors (metabotropic, kainate, NMDA, and AMPA).

SAFETY INFORMATION

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves