Glucose (HK) Assay Kit ยี่ห้อ Sigma

PROPERTIES

usage

sufficient for 20 assays

Quality Level

100

analyte chemical class(es)

sugars (glucose)

technique(s)

photometry: suitable

application(s)

food and beverages

general analytical

storage temp.

2-8°C

DESCRIPTION

Application

This kit is for the quantitative, enzymatic determination of glucose in food and other material. Glucose is phosphorylated by adenosine triphosphate (ATP) in the reaction catalyzed by hexokinase. Glucose-6-phosphate (G6P) is then oxidized to 6-phospho-gluconate in the presence of oxidized nicotinamide adenine dinucleotide (NAD) in a reaction catalyzed by glucose-6-phosphate dehydrogenase (G6PDH). During this oxidation, an equimolar amount of NAD is reduced to NADH. The consequent increase in absorbance at 340 nm is directly proportional to glucose concentration.