BMP-2 (human) ยี่ห้อ Sigma

Properties

biological source

human

Quality Level

100

recombinant

expressed in HEK 293 cells

form

lyophilized powder

potency

≤60 ng/mL EC₅₀

quality

endotoxin tested

mol wt

dimer 30-38 kDa (glycosylated)

packaging

pkg of 10 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

≤1 EU/μg Endotoxin level

UniProt accession no.

P12643

storage temp.

−20°C

Gene Information

human ... BMP2(650)

Description

General

description

Bone Morphogenetic Protein 2 is a member of the TGF-β

superfamily of cytokines that affect bone and cartilage formation. It is

important for skeletal development during embryogenesis. BMP-2 induces

chondrocyte formation, osteoblast differentiation, and is involved in embryo

dorsal-ventral patterning and organogenesis.

Biochem/physiol

Actions

It has been reported that Bone Morphogenetic Protein 2 (BMP-2)

inhibits estradiol induced proliferation of human breast cancer cells. BMP-2

signaling mediates apoptosis by activation of the TAK1-p38 kinase pathway that

is negatively regulated by Smad6. Cellular responses to BMP-2 are mediated by

the formation of hetero-oligomeric complexes of type I and type II

serine/threonine kinase receptors, which play significant roles in BMP binding

and signaling. One BMP type II receptor and two BMP type I receptors have been

identified. Both BMP type I receptors bind BMP-2 with high-affinity in the

absence of BMP receptor type II.

Physical form

Lyophilized from a 0.2 μm filtered solution of 2x PBS + 6%

Ethanol.

Preparation Note

This Bone Morphogenetic Protein 2 (BMP-2) is produced from a DNA

sequence encoding the human BMP-2 protein, expressed in HEK 293 cells. It is a

glycosylated homodimer linked by a single disulfide bond with an apparent

molecular mass of 30-38 kDa.

Analysis Note

The specific activity was determined by its ability to induce

alkaline phosphatase production in a dose response to BMP-2 in the ATDC-5 cell

line (mouse chondrogenic cell line).

Legal Information

HumanKine is a registered trademark of Proteintech Group, Inc.

and Humanzyme, Inc

Safety Information

WGK

WGK 3