Monoclonal Anti-HA−Peroxidase antibody produced in mouse, clone HA-7, purified immunoglobulin, lyophilized powder ยี่ห้อ Sigma

PROPERTIES

biological source

mouse

Quality Level

200

conjugate

peroxidase conjugate

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

HA-7, monoclonal

form

lyophilized powder

packaging

vial of 0.5 mL

concentration

5-11 mg/mL

technique(s)

western blot: 1:4,000 using using HA tagged fusion protein expressed in bacteria, and ECL immunoblotting detection reagent

storage temp.

2-8°C

DESCRIPTION

General description

Influenza A virus, a RNA virus, encodes two surface glycoproteins, hemagglutinin (HA) and neuraminidase (NA).[1] HA stalk domain based epitope region antibody is effective in neutralizing divergent influenza virus strains.[2]

The mouse monoclonal Anti-HA-Peroxidase antibody recognizes native and denatured-reduced forms of the HA-tagged proteins. It also reacts with the N- or C-terminal HA-tagged fusion proteins expressed in E. coli and mammalian cells.

Specificity

The antibody recognizes native as well as denatured-reduced forms of HA-tagged proteins and is reactive with N- or C-terminal HA-tagged fusion proteins expressed in E. coli or in mammalian cells.

Immunogen

synthetic peptide corresponding to a fragment of human influenza virus hemagglutinin (HA) known as HA-tag, conjugated to KLH

Application

Monoclonal Anti-HA-Peroxidase antibody produced in mouse has been used:

·       in immunopreciptation and immunostaining of human member 9 of solute carrier family 38 SLC38A9 transfected in HeLa cells[3]

·       in immunoblot detection in transfected A375 cells[4]

·       is suitable for immunoblotting of nucleosome assembly protein NAP1L1[5]

Physical form

Lyophilized from 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.05% MIT.

Preparation Note

Reconstitute in .5mL of distilled water.