3-Hydroxy-3-methylglutaryl-CoA reductase human solution ยี่ห้อ Sigma

PROPERTIES

Assay

≥90% (SDS-PAGE)

Quality Level

200

form

solution

specific activity

2-8 units/mg protein

mol wt

~76 kDa (for the fusion protein)

UniProt accession no.

P04035

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... HMGCR(3156)

DESCRIPTION

Application

3-Hydroxy-3-methylglutaryl-CoA reductase human has been used:

in anti-HMGCR enzyme linked immunosorbent assay (ELISA)[1]

as an antigen to measure anti-HMGCR antibodies by blot analysis[2]

in HMG-CoA reductase activity assays[3]

as an antigen to compare in-house antigen via sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE)/western blot[4]

Biochem/physiol Actions

3-Hydroxy-3-methylglutaryl-CoA reductase/HMGCR serves as a rate limiting enzyme in mevalonate pathway. It also controls the oncogenic Hippo transducers TAZ/YAP.[5] HMGCR also perform as a genetic modifier for Alzheimer′s disease (AD).[6]

Hydroxy-3-methylglutaryl-CoA reductase catalyzes the committed step in cholesterol biosynthesis.

Physical properties

Consists of the HMG CoA reductase catalytic domain fused to GST protein.

Unit Definition

One unit will convert 1.0 μmole of NADPH to NADP+ per minute at 37 °C.

Physical form

Supplied as a solution containing 250 μg protein in 50 mM Tris pH 7.5, with 5 mM DTT, 1:200 Protease Inhibitor Cocktail (Catalog Number P8340) and 50% (w/v) glycerol.

SAFETY INFORMATION

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable