Transforming Growth Factor-β1 human ยี่ห้อ Sigma

รหัสสินค้า : H8541

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PROPERTIES

biological source

human

Quality Level

300

recombinant

expressed in HEK 293 cells

Assay

≥95% (SDS-PAGE)

form

lyophilized powder

potency

≤0.5 ng/mL EC₅₀

quality

endotoxin tested

mol wt

dimer 25 kDa (non-glycosylated)

packaging

pkg of 5 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

≤1 EU/μg

UniProt accession no.

P01137

storage temp.

-10 to -25°C

Gene Information

human ... TGFB1(7040)

DESCRIPTION

General description

The TGFB1 gene is mapped to human chromosome 19q13.2 and codes for a 25 kDa peptide.[1][2] TGF-ß1 polypeptide is produced as a precursor with hydrophobic signal sequence, pro-region and mature peptide. Cleavage of the precursor at tetrabasic cleavage site results in monomers of carboxy-terminal 112 amino acids. The biologically active form of Tgfb1 exists as a disulphide-linked homodimer.[3]

Application

Transforming Growth Factor-β1 (TGF-β) human has been used :

· to study the expression of PDGFRB (Platelet derived growth factor receptor β) in association with transforming growth factor-β signaling.[4]

· to stimulate lung fibrosis in human fetal lung fibroblast cell line in order to further measure the in-plane elasticity of live cell layers using a pressure sensor embedded microfluidic device.[5]

· to study the effect of TGF-β on HTRA1 (high-temperature requirement A1)-affected Wnt target genes.[6]

Biochem/physiol Actions

TGF-β1 is a multifunctional peptide capable of influencing cell proliferation, differentiation, and other functions in a wide range of cell types.[2] Transformed as well as non-neoplastic tissues release transforming growth factors and essentially all cells possess a specific TGF-β1 receptor. In general, cells of mesenchymal origin appear to be stimulated by TGF-β1; whereas, hepatocytes, T and B lymphocytes, keratinocytes, and many epithelial cells are inhibited by the peptide. TGF-β1 interacts with Epidermal Growth Factor (EGF), Platelet Derived Growth Factor (PDGF), Fibroblast Growth Factor (FGF), and T Cell Growth Factor (T-CGF) either by enhancing or antagonizing their characteristic actions. TGF-β1 plays a fundamental role in tissue growth and differentiation by involvement in adipogenesis, myogenesis, chondrogenesis, osteogenesis, epithelial cell differentiation, and immune cell function.[7] TGF-β1 is associated with the developmental and metastasis of colorectal cancer.[8]

Transforming growth factor-β1 (TGF-β1) is produced by many cell types, but is reported to be most concentrated in mammalian platelets, where it is present at approximately four times the level of TGF-β2.

Physical form

Lyophilized from a 0.2 μm filtered solution of 50 mM NaOAc pH 4.5

Analysis Note

The activity was determined by the dose dependent inhibition of IL-4 induced proliferation of mouse HT-2 cells (BALB/c spleen activated by sheep erythrocytes in the presence of IL-2).

SAFETY INFORMATION

Pictograms