Immobilon®-P PVDF Transfer Membrane, 0.45 μm, 7 x 8.4 cm 50/pk ยี่ห้อ MERCK

PROPERTIES

material

PVDF membrane

plain filter

white filter

Quality Level

400

feature

hydrophobic

manufacturer/tradename

Immobilon^®

technique(s)

western blot: suitable

filter L × W

7 cm × 8.4 cm

pore size

0.45 μm pore size

capacity

160 μg/cm² adsorption capacity (insulin)

215 μg/cm² adsorption capacity (BSA)

294 μg/cm² adsorption capacity (goat IgG)

compatibility

for use with Amido black

for use with CPTS

for use with Colloidal gold

for use with Coomassie brilliant blue

for use with India ink

for use with Ponceau-S red

for use with Sypro<TMSYMBOL></TMSYMBOL> ruby

for use with Toluidine blue

for use with Transillumination

detection method

chemiluminescent

colorimetric

radioactive

shipped in

ambient

DESCRIPTION

General description

Immobilon^®-P transfer membrane is a polyvinylidene fluoride (PVDF) microporous membrane with a nominal pore size of 0.45 micron (μm). It is recommended for western blotting, especially for proteins greater than 20 kDa. Immobilon^®-P transfer membrane can bind a wide range of molecular weight proteins. It is used for the transfer of proteins from a variety of gel matrices. When compared to a nitrocellulose membrane, the Immobilon-P PVDF membrane has a higher signal-to-noise ratio for enhanced sensitivities, improved handling characteristics and staining capabilities and increased solvent resistance.

Primary Binding Mechanisms: Electrostatic, hydrophobic;

Filter Code: IPVH

Application

Immobilon^®-P PVDF Membrane has been used in western blotting.[1][2][3]

Features and Benefits

Immobilon^®-P membrane has:

·       excellent protein retention

·       high physical strength

·       broad chemical compatibility

·       suitable for a variety of staining applications and reprobing in immunodetection methods

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

SAFETY INFORMATION

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable