InstantBlue™, Ultrafast Protein Stain ยี่ห้อ Sigma

PROPERTIES

Quality Level

100

manufacturer/tradename

ISBT

technique(s)

protein staining: suitable

storage temp.

2-8°C

DESCRIPTION

General description

InstantBlue^™ is a ready-to-use solution stain that is specially formulated for ultra-fast (less than 15 min), sensitive (5 ng per band (BSA)) and safe detection of your proteins. Protein gels can be stained with InstantBlue in minutes without the need to wash, fix or destain. Gels can remain in the stain for weeks without concern. Only the proteins are stained resulting in well-defined blue bands on a highly transparent background. The reduction of background interference results in a better signal to noise ratio and may also have a positive impact on the overall resolution and sensitivity. The InstantBlue formulation is non-toxic and does not contain any methanol. Proteins stained using the InstantBlue stain are also compatible with mass spectrometry (MS) analysis.

Application

InstantBlue^™ has been used in coomassie staining to validate the presence of proteins[1] from SDS-PAGE gels.[2] It has also been used for staining SDS-PAGE gels.[3]

Components

contains Coomassie dye, ethanol, phosphoric acid and solubilizing agents in water. (Caution: Phosphoric acid is a corrosive liquid.)

Other Notes

Not available in Sweden and Denmark

Legal Information

InstantBlue is a trademark of Expedeon Protein Solutions