N,N'-Methylenebisacrylamide solution, for electrophoresis, 2% in H₂O ยี่ห้อ Sigma

PROPERTIES

sterility

0.2 μm filtered

Quality Level

200

form

liquid

concentration

2% in H₂O

technique(s)

electrophoresis: suitable

storage temp.

2-8°C

SMILES string

C=CC(=O)NCNC(=O)C=C

InChI

1S/C7H10N2O2/c1-3-6(10)8-5-9-7(11)4-2/h3-4H,1-2,5H2,(H,8,10)(H,9,11)

InChI key

ZIUHHBKFKCYYJD-UHFFFAOYSA-N

DESCRIPTION

General description

N,N′-Methylenebisacrylamide solution is a cross-linking agent used in polyacrylamide gel preparation.[1]

Application

N,N′-Methylenebisacrylamide solution has been used as a component of hydrogel precursor solution and magnified analysis of the proteome (MAP) gel solution during hydrogel fabrication.[2][3] It has also been used as a component of re-embedding solution during re-embedding of expanded hydrogels.[4]

To be mixed with acrylamide for making polyacrylamide gels for use in protein and nucleic acid electrophoresis.

Preparation Note

Prepared from electrophoresis grade bis-acrylamide in ultra-pure water.