N,N'-Methylenebisacrylamide, powder, for molecular biology, for electrophoresis, ≥99.5% ยี่ห้อ Sigma

PROPERTIES

grade

for molecular biology

Quality Level

200

Assay

≥99.5%

form

powder

technique(s)

electrophoresis: suitable

impurities

≤0.05% Free carboxylic acid (T)

≤50 mg/kg Total sulfur (as SO₄ ) (ICP)

conductivity

≤10 μmho (2% solution)

anion traces

chloride (Cl^-): ≤50 mg/kg

cation traces

Ca: ≤10 ppm

Cd: ≤5 ppm

Co: ≤5 ppm

Cr: ≤5 ppm

Cu: ≤5 ppm

Fe: ≤5 ppm

K: ≤50 ppm

Mg: ≤5 ppm

Mn: ≤5 ppm

Na: ≤50 ppm

Ni: ≤5 ppm

Pb: ≤5 ppm

Zn: ≤5 ppm

absorption

≤0.2 at 290 at 1%

foreign activity

DNase and RNase, none detected

SMILES string

C=CC(=O)NCNC(=O)C=C

InChI

1S/C7H10N2O2/c1-3-6(10)8-5-9-7(11)4-2/h3-4H,1-2,5H2,(H,8,10)(H,9,11)

InChI key

ZIUHHBKFKCYYJD-UHFFFAOYSA-N

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DESCRIPTION

General description

N,N′-Methylenebisacrylamide is commonly called as bis. It is used as a cross-linking agent in the preparation of polyacrylamide gels consisting of chains of acrylamide monomers. The concentration of N,N′-methylenebisacrylamide and acrylamide determines the pore size of the gel. The higher concentration of bis forms rigid gels with smaller pores due to more cross-linking leading to polymerization and vice versa.[1]

Application

N,N′-Methylenebisacrylamide has been used:

·       as a crosslinker in the monomer solution to prepare N,N-dimethyl acrylamide (DMAAm)-based cryogel and acrylamide (AAm)-based cryogel matrix[2]

·       as a crosslinker to prepare tough hydrogel network[3]

·       in the preparation of acrylamide-bisacrylamide stock solution for the preparation of polyacrylamide gel[4]

To be mixed with acrylamide for making polyacrylamide gels for use in protein and nucleic acid electrophoresis.