ANTI-FLAG® M2 Magnetic Beads ยี่ห้อ MERCK

PROPERTIES

conjugate

magnetic beads

Quality Level

200

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

M2, monoclonal

form

suspension

shelf life

2 yr at -20 °C

analyte chemical class(es)

proteins (FLAG^® Affinity Gels, FLAG^® tag, 3x FLAG^® tag, DYKDDDDK tag)

technique(s)

affinity chromatography: suitable

immunoprecipitation (IP): suitable

bead size

20-75 μm

matrix

superparamagnetic iron impregnated 4% agarose bead, with an average diameter of 50 μm.

isotype

IgG1

capacity

≥0.6 mg/mL binding capacity

shipped in

wet ice

storage temp.

−20°C

DESCRIPTION

General description

Anti-FLAG M2 Magnetic Beads are 4% agarose beads bound with the Anti-FLAG M2 (mouse monoclonal) antibody. The M2 antibody recognizes the FLAG sequence at the N-terminus, Met-N-terminus and C-terminus. This alows for detection and capture of fusion proteins containing a FLAG peptide sequence.

Application

Suitable for immunoprecipitation procedures.

Elution - FLAG^® peptide, Glycine, pH 3.5, 3x FLAG^® peptide

Browse additional application references in our FLAG^® Literature portal.

Features and Benefits

The magnetic properties allow for:

- Very rapid separation

- Significantly accelerated manipulations, such as repetitive washings

- Processing of multiple samples performed in plate formats

This leads to:

- Faster experimentation

- Better reproducibility

- More accurate quantitation of the proteins of interest

Physical form

Supplied as a 50% suspension in 50% glycerol with 10mM sodium phosphate, 150mM sodium chloride, pH 7.4 and 0.02% (w/v) sodium azide (PBA/A).

Other Notes

Do not use magnetic stirring system as this will destroy the resin beads.

Legal Information

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC

FLAG is a registered trademark of Sigma-Aldrich Co. LLC