Anti-Rabbit light chain, HRP conjugate Antibody; produced in mouse; Purified from antiserum by chromatographic procedures. Lyophilized in 0.01M Sodium Phosphate, ยี่ห้อ Sigma

PROPERTIES

biological source

mouse

Quality Level

100

conjugate

peroxidase conjugate

antibody form

purified antibody

antibody product type

secondary antibodies

clone

monoclonal

species reactivity

rabbit

manufacturer/tradename

Chemicon^®

technique(s)

ELISA: suitable

immunocytochemistry: suitable

immunohistochemistry: suitable

western blot: suitable

isotype

IgG1κ

shipped in

wet ice

DESCRIPTION

General description

Antibody molecules typically comprise two immunoglobulin light chains covalently bound to a pair of heavy chains. Immunoglobulin light chains occur in two types, designated by the Greek letters kappa and lambda. Kappa and gamma light chains are approximately 250 amino acids in length with an average mass of about 25 kDa. The ratio of kappa to lambda found in the immunoglobulin population varies by species.

Specificity

Minimal cross-reaction with bovine, goat, Armenian hamster, horse, human, mouse, rat, and sheep.

The antibody reacts strongly with native primary antibodies primarily with kappa light chains. It is not suitable for detecting lambda light chains. The antibody does not react with the heavy chain of rabbit IgG. The antibody has been tested by ELISA and adsorbed to ensure minimal cross-reaction with bovine, goat, Armenian hamster, horse, human, mouse, rat, and sheep immunoglobulins.

Immunogen

Epitope: Kappa light chain

Prepared from rabbit IgG light chain.

Application

Detect Rabbit light chain using this Mouse anti-Rabbit light chain, HRP conjugate Antibody validated for use in ELISA, WB, IC, IH.

Research Category

Secondary & Control Antibodies

Research Sub Category

Fragment Specific Secondary Antibodies

Western Blotting: 1:5,000 -1:100,000 with chromogenic substrates 1:10,000 -1:200,000 with ECL substrates

ELISA: 1:5,000 -1:100,000

Immunohistochemistry: 1:500 - 1:5,000

Immunocytochemistry: 1:500 - 1:5,000

Optimal working dilutions must be determined by the end user.

Target description

25 kDa

Physical form

Purified from antiserum by chromatographic procedures. Lyophilized in 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6. 15 mg/mL BSA as stabilizer.

Serum

Storage and Stability

Maintain lyophilized product at 2°-8°C for up to 12 months. After reconstitution the product is stable for six weeks at 2°-8°C in the dark as an undiluted liquid. Reconstitute vial with of distilled water (addition of 0.5 ml water will yield a final concentration of 1 mg/mL). For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze thaw cycles. Keep away from light.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

SAFETY INFORMATION

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable