Glutamate Assay Kit ยี่ห้อ Sigma

PROPERTIES

usage

sufficient for 100 colorimetric tests

detection method

colorimetric

storage temp.

−20°C

DESCRIPTION

General description

Glutamate, one of the two acidic proteinogenic amino acids, is also a key molecule in cellular metabolism.[1] The synthesis of α-KG from glutamate by glutamate dehydrogenase is a key TCA cycle anaplerotic reaction. In humans, glutamate plays an important role both in amino acid degradation and disposal of excess or waste nitrogen.[2] Within the mammalian nervous system, glutamate is the most abundant excitatory neurotransmitter.[1] It is believed to be involved in learning and memory and may also be involved in diseases like amyotrophic lateral sclerosis, autism, some forms of mental retardation, and Alzheimer′s disease.[3] Glutamate is present in a wide variety of foods, and has been used as a flavor enhancer in food industry.

Application

Glutamate Assay Kit has been used to determine the concentration of glutamate in cerebrospinal fluid in a rat subarachnoid hemorrhage model.[4] It has also been used to determine cytosolic and secreted glutamate concentrations in cerebrospinal fluid of patients with amyotrophic lateral sclerosis.[5]

Suitability

Suitable to determine glutamate concentration in a variety of samples.

Principle

Glutamate concentration is determined by an enzymatic assay, which results in a colorimetric (450 nm) product, proportional to the glutamate present.