Phenylalanine Assay Kit ยี่ห้อ Sigma

PROPERTIES

usage

sufficient for 100 fluorometric tests

detection method

fluorometric

storage temp.

−20°C

DESCRIPTION

General description

Phenylalanine is a non-polar essential amino acid.[1][2] In the liver, phenylalanine is converted to tyrosine, which is a precursor for multiple compounds including L-DOPA[3] and melanin. Defects in the activity of phenylalanine hydroxylase result in the inherited metabolic disorder phenylketonuria (PKU). PKU results in the build up of phenylalanine and phenylalanine metabolites, and can result in growth defects and mental retardation if not treated.[4]

Suitability

Suitable for L-phenylalanine detection in cell and tissue culture supernatants, serum, and other biological samples

Principle

Phenylalanine concentration is determined by a coupled enzyme assay, which results in the deamination of phenylalanine and the production of NADH which reacts with the probe resulting in a fluorescent (λex = 535 nm/λem = 587 nm) product, proportional to the phenylalanine present.