Hydroxyproline Assay Kit ยี่ห้อ Sigma

PROPERTIES

usage

sufficient for 100 colorimetric tests

detection method

colorimetric

shipped in

wet ice

storage temp.

2-8°C

DESCRIPTION

General description

Hydroxyproline (4-hydroxyproline) is a non-proteinogenic amino acid[1] formed by the post-translational hydroxylation of proline. Hydroxyproline is a major component of collagen, where it serves to stabilize the helical structure.[2] Because hydroxyproline is largely restricted to collagen, the measurement of hydroxylproline levels can be used as an indicator of collagen content. Conditions that increase collagen turnover can elevate serum and urine hydroxyproline levels. Urine and serum hydroxyproline levels can be used as a marker for bone resorption.[3][4]

Application

Hydroxyproline Assay Kit has been used to determine the concentration of hydroxyproline and collagen.[5][6][7]

Suitability

Suitable for hydroxyproline detection in cell and tissue culture supernatants, urine, plasma, serum, and other biological samples.

Principle

In the Hydroxyproline Assay Kit, hydroxyproline concentration is determined by the reaction of oxidized hydroxyproline with 4-(Dimethylamino)benzaldehyde (DMAB), which results in a colorimetric (560 nm) product, proportional to the hydroxyproline present.