Maltose and Glucose Assay Kit ยี่ห้อ Sigma
รหัสสินค้า : MAK018
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PROPERTIES
usage
sufficient for 100 colorimetric or fluorometric tests
detection method
colorimetric
fluorometric
storage temp.
−20°C
DESCRIPTION
General description
Maltose is a disaccharide containing two glucose molecules with an a(1→4) glycosidic linkage. The enzyme α-D-glucosidase hydrolyzes maltose to its constituent monosaccharides. Glucose is the primary carbohydrate utilized for energy during cellular respiration. Maltose can be derived from starch in food through the action of amylase. Maltose can be found in many food products, including beer, cereals, and pasta.
Application
Maltose and Glucose Assay Kit has been used to estimate maltose present in coral, coral larvae, Symbiodinium and seawater.[1]
Suitability
Suitable for the direct detection of maltose and glucose in a variety of samples, including serum, plasma, food, or growth media.
Principle
The Maltose and Glucose Assay kit provides a simple and direct procedure for measuring maltose and glucose in a variety of samples, including serum, plasma, food, or growth media. In this kit, maltose is converted to two glucose units via α-D-Glucosidase. Glucose is further oxidized, resulting in a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the maltose present. This kit has a linear detection range of 0.1-0.5 nmole maltose for the fluorometric assay and 1-5 nmoles maltose for the colorimetric assay.
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