Chloride Assay Kit ยี่ห้อ Sigma

PROPERTIES

usage

sufficient for 100 colorimetric tests

detection method

colorimetric

storage temp.

room temp

DESCRIPTION

General description

Chloride is an essential anion needed for the proper functioning of many critical aspects of metabolism, including aiding in maintenance of the body′s acid-base balance. In Cystic Fibrosis, mutations to the CF transmembrane conductance regulator (CFTCR) gene result in altered sodium and chloride ion transport channels.[1]

Application

Chloride Assay Kit has been used to measure chloride concentration spectrophotometrically and is used to measure chloride levels in blood plasma of toads Rhinella marina.[2][3][1]

Suitability

Suitable for the quantification of Chloride in a variety of biological samples. Blood and urine can be used directly after dilution with water

Principle

The Chloride Assay kit provides a simple and direct procedure for measuring chloride in a variety of samples, including blood and urine. Chloride concentration is determined by a competition reaction between Hg2+ and Fe2+ for 2,4,6-Tris(2-pyridyl)-s-triazine(TPTZ). The preferred Hg-TPTZ complex exhibits no color. In the presence of chloride, Hg2+ forms HgCl2, which precipitates, allowing TPTZ to complex with Fe2+. The Fe-TPTZ complex results in a colorimetric (620 nm) product proportional to the chloride present.

SAFETY INFORMATION

Hazard Statements

H412

Precautionary Statements

P273 - P501

Hazard Classifications

Aquatic Chronic 3

Storage Class Code

10 - Combustible liquids

WGK

WGK 2