Cobalt Assay ยี่ห้อ Sigma

PROPERTIES

usage

sufficient for 100 colorimetric tests

detection method

colorimetric

storage temp.

room temp

DESCRIPTION

General description

Cobalt is a transition metal that serves as a trace dietary mineral for all multicellular organisms. Cobalt is an important cofactor for the Vitamin B12 class of compounds where it occupies the center of the vitamin B12 corrin ring.[1] Cobalt can also be coordinated in the active site of the non-corrin containing metalloenzyme methionine aminopeptidase.[1]

Suitability

Suitable for quantitating cobalt concentrations in a variety of samples

Principle

The Cobalt Assay kit provides a simple and direct procedure for measuring cobalt in a variety of samples. In this assay, cobalt reacts with 2-mercaptoethanol under basic conditions to form a complex with a strong absorbance at 475 nm. Interference from the metal ions Fe2+, Cu2+, Ni2+, Zn2+, and Mn2+ is <10% at this wavelength. This assay gives a linear range of 10-50 nmoles of cobalt.