Nickel Assay Kit ยี่ห้อ Sigma

PROPERTIES

usage

sufficient for 100 colorimetric tests

detection method

colorimetric

storage temp.

2-8°C

DESCRIPTION

General description

Nickel acts as a cofactor for several enzymes, including some ureases, carbon monoxide dehydrogenases (methane forming enzymes which reduce CO2 to CH4), and some hydrogenases. Nickel forms complexes with sulfhydryl compounds with significant absorbance in the UV/visible region in the presence of other ions.

In the Nickel Assay kit, Ni2+ reacts with 2-mercaptoethanol in borate buffer to form a complex with strong absorbance bands from 300 to 600 nm. Fe2+ and Co2+ interfere with the assay; therefore, extra steps must be taken to subtract the interference in order to determine the correct nickel concentration in mixed samples. Other ions tested (Mn2+, Cu2+, and Zn2+) do not interfere with the assay and presumably no other ionic species will be present in high enough concentration to interfere with the reaction. The assay is a simple method of quantifying Ni2+ in a variety of samples, which gives a linear range of 2–50 nmole nickel in samples containing <25 nmole of Co2+ ions.

Nickel acts as a cofactor for several enzymes, including some ureases, carbon monoxide dehydrogenases (methane forming enzymes which reduce CO2 to CH4), and some hydrogenases.[1] Nickel forms complexes with sulfhydryl compounds with significant absorbance in the UV/visible region in the presence of other ions.

Suitability

Suitable for quantifying nickel concentrations in a variety of samples.

Principle

In the Nickel Assay kit, Ni2+ reacts with 2-mercaptoethanol in borate buffer to form a complex with strong absorbance bands from 300 to 600 nm. Fe2+ and Co2+ interfere with the assay; therefore, extra steps must be taken to subtract the interference in order to determine the correct nickel concentration in mixed samples. Other ions tested (Mn2+, Cu2+, and Zn2+) do not interfere with the assay and presumably no other ionic species will be present in high enough concentration to interfere with the reaction. The assay is a simple method of quantifying Ni2+ in a variety of samples, which gives a linear range of 2–50 nmole nickel in samples containing <25 nmole of Co2+ ions.