Hemin Assay Kit, ยี่ห้อ Sigma

DESCRIPTION

General description

Hemin, the oxidized version of heme, is an iron-containing prosthetic group for a diverse group of proteins. Free heme, which can be released from hemoglobin following hemolysis, is pro-inflammatory and contributes to iron-derived reactive oxygen species. Free hemin levels may be upregulated in various pathological conditions and can contribute to various inflammatory conditions including vascular disorders, renal failure, and immune-mediated disorders.

Application

Hemin Assay Kit has been used to determine the level of free hemin (oxidized heme).

Suitability

Suitable for use with cell and tissue culture supernatants, urine, plasma, serum, fecal material, media, and other biological fluids.

Principle

In this assay, Hemin concentration is determined by a coupled enzyme reaction, which results in a colorimetric (570 nm) product, proportional to the Hemin present.