Lipase activity assay kit ยี่ห้อ Sigma

PROPERTIES

usage

sufficient for 100 colorimetric tests (with glycerol standard)

detection method

colorimetric

storage temp.

−20°C

DESCRIPTION

General description

The lipase family of enzymes catalyze the cleavage of the ester bonds of lipids. In mammals, this family includes many critical members including pancreatic, hepatic, endothelial, and lipoprotein lipase. Lipases, such as pancreatic lipase, are critical for the metabolism of lipids. Lipases also play key roles in processes such as cell signaling and inflammation. Measurements of lipase activity are commonly used to screen for pancreatic injury or disease, and to monitor diseases such as cystic fibrosis, celiac disease, and Crohn′s disease.

Application

Lipase Activity Assay Kit has been used to determine the lipase activity in samples.[1][2]

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the detection of lipase activity in biological samples including tissue, cells and serum

Principle

The Lipase Activity Assay kit provides a simple and direct procedure for measuring lipase activity in a variety of samples. Lipase activity is determined using a coupled enzyme reaction, which results in a colorimetric (570 nm) product proportional to the enzymatic activity present. One unit of Lipase is the amount of enzyme that will generate 1.0 μmole of glycerol from triglycerides per minute at 37 °C.