Phosphatidylcholine assay kit ยี่ห้อ Sigma

PROPERTIES

usage

sufficient for 100 colorimetric or fluorometric tests

detection method

colorimetric

fluorometric

storage temp.

−20°C

DESCRIPTION

General description

Phosphatidylcholine (PC) accounts for more than 50% of the phospholipids composing mammalian plasma membranes and is particularly enriched in the extracellular leaflet. PC is synthesized in the liver and is required for lipoprotein assembly and secretion. PC is cleaved by phospholipase D, which hydrolyses the choline head, producing the signaling intermediate phosphatidic acid.

Application

Phosphatidylcholine Assay Kit has been used to determine the concentration of phosphatidylcholine in samples.[1][2][3]

Suitability

Suitable for the measurement of phosphatidylcholine in a variety of biological samples

Principle

In this assay, PC concentration is determined by a coupled enzyme reaction, which results in a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the PC present.