Sarcosine Assay Kit ยี่ห้อ Sigma

PROPERTIES

usage

sufficient for 100 colorimetric or fluorometric tests

detection method

colorimetric

fluorometric

storage temp.

−20°C

DESCRIPTION

General description

Sarcosine, also known as N-methylglycine, is an amino acid that participates in one-carbon metabolism, serving as a one-carbon donor in mitochondrial reactions. Elevated levels of sarcosine may indicate deficiencies in Vitamin B12 and/or folate. Sarcosine is also an intermediate in glycine and choline metabolism. In the brain, sarcosine acts as an inhibitor of the type I glycine transporter, resulting in increased glycine levels and NMDA receptor activation suggesting it may be a therapeutic agent for the treatment of some mental illnesses such as schizophrenia, depression, or obsessive compulsive disorder.

Suitability

This kit is suitable for sarcosine detection in cell and tissue culture supernatants, plasma, serum, and other biological samples. Urine samples are not suitable for use with this assay because of sample interferences.

Principle

In this assay, sarcosine concentration is determined by a coupled enzyme reaction, which results in a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the sarcosine present.

SAFETY INFORMATION

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

188.6 °F - closed cup

Flash Point(C)

87 °C - closed cup