Ascorbic Acid Assay Kit ยี่ห้อ Sigma
รหัสสินค้า : MAK074
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PROPERTIES
usage
sufficient for 100 colorimetric or fluorometric tests
detection method
colorimetric
fluorometric
storage temp.
−20°C
DESCRIPTION
General description
Ascorbic Acid, also known as Vitamin C, is a six-carbon lactone produced by plants and some animal species but not by humans and other primates. Ascorbic acid functions as an enzymatic cofactor for multiple enzymes, serving as an electron donor for monooxygenases and dioxygenases. Ascorbic acid also functions as a powerful antioxidant, particularly in regards to reactive oxygen species.
Suitability
Suitable for use with biological fluids, food samples, and growth media. Proteins present in the sample can interfere with this assay. Protein containing samples are best analyzed using the Ascorbic Acid Assay II (MAK075). Alternatively, samples can be deproteinized using a 10 kDa Molecular Weight Cut-Off Spin Filter.
Principle
In this assay, Ascorbic Acid concentration is determined by a coupled enzyme reaction, which results in a colorimetric (570 nm)/fluorometric (λex = 535/ λem = 587 nm) product, proportional to the ascorbic acid present.
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