Ascorbic Acid Assay Kit II ยี่ห้อ Sigma

PROPERTIES

usage

sufficient for 100 colorimetric tests

detection method

colorimetric

storage temp.

−20°C

DESCRIPTION

General description

Ascorbic Acid, also known as Vitamin C, is a six-carbon lactone produced by plants and some animal species but not by humans and other primates. Ascorbic acid functions as an enzymatic cofactor for multiple enzymes, serving as an electron donor for monooxygenases and dioxygenases. Ascorbic acid also functions as a powerful antioxidant, particularly in regards to reactive oxygen species.

Application

Ascorbic Acid Assay Kit II has been used to determine ascorbic acid concentration in vitreous sample from the eye.[1]

Suitability

Suitable for use with cell and tissue culture supernatants as well as serum, plasma, urine, and other biological fluids.

Principle

In this assay, ascorbic acid concentration is determined using the Ferric Reducing/Antioxidant and Ascorbic Acid (FRASC) assay. Fe3+ is reduced to Fe2+ by antioxidants present in the sample, which results in a colorimetric (593 nm) product. The addition of ascorbate oxidase to parallel samples oxidizes any ascorbic acid present allowing for the measurement of the ascorbic acid concentration.

SAFETY INFORMATION

Storage Class Code

10 - Combustible liquids

WGK

WGK 3