Adp colorimetric assay kit ii ยี่ห้อ Sigma

PROPERTIES

usage

sufficient for 100 colorimetric tests

detection method

colorimetric

storage temp.

−20°C

DESCRIPTION

General description

Adenosine diphosphate (ADP) is a nucleoside diphosphate that plays a critical role in energy transfer reactions.[1] ADP is produced from adenosine triphosphate via the action of ATPases. ADP also plays a critical role in platelet function.[2] ADP, stored in platelet-dense granules, is released upon platelet activation where it acts on purinergic receptors to mediate intracellular signaling and platelet aggregation.[3][4]

Application

ADP Colorimetric Assay Kit II has been used to measure the ADP level in infusion medium and variety of samples, particularly those containing reducing agents that may interfere with oxidase based assays.[5][6]

Suitability

Suitable for measuring ADP levels in samples that contain reducing substances, which may interfere with oxidase-based assays. Samples include tissue (liver, muscle, heart etc.) and cell culture (adherent or suspension cells).

Principle

The ADP Assay kit provides a simple and direct procedure for measuring ADP in a variety of samples. ADP concentration is determined by a coupled enzyme assay, which results in a colorimetric (450 nm) product proportional to the ADP present.