Gamma Glutamyl Transferase (GGT) Activity Fluorometric Assay Kit, ยี่ห้อ Sigma

PROPERTIES

usage

sufficient for 100 fluorometric tests

detection method

fluorometric

storage temp.

−20°C

Gene Information

human ... GGT1(2678) , GGT2(728441) , GGT5(2687) , GGT6(124975) , GGT7(2686)

mouse ... GGT1(14598) , GGT5(23887) , GGT6(71522) , GGT7(207182)

rat ... GGT1(116568) , GGT5(296566) , GGT6(408206) , GGT7(156275)

DESCRIPTION

General description

γ-Glutamyltransferase (GGT, EC 2.3.2.2) is a membrane-bound protein that catalyzes the transfer of γ-glutamyl moieties to acceptor molecules such as amino acids or peptides. GGT plays a key role in the γ-glutamyl cycle, a critical pathway for glutathione homeostasis as well as the detoxification of xenobiotics. Serum GGT levels can be elevated in many pathophysiological conditions such as cardiovascular disease, chronic liver disease, and metabolic syndrome. Elevated serum levels of GGT can also be indicative of oxidative stress.[1]

Application

γ-Glutamyltransferase (GGT) Activity Fluorometric Assay Kit has been used to analyze serum GGT activity.[2][3]

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the measurement of gamma-glutamyl transferase (GGT) in a variety of samples including tissue, cells and serum

Principle

The GGT Activity Fluorometric Assay kit provides a simple and direct procedure for measuring GGT activity in a variety of samples. GGT activity is determined by a coupled enzyme assay, in which the GGT transfers the γ-glutamyl group from quenched substrate L-γ-Glutamyl-7-amido-4-methylcoumarin (L--Glutamyl-AMC), liberating the fluorescent group, 7-Amino-4-Methyl Coumarin (AMC) (λ_ex = 365 nm/λ_em = 460 nm) proportional to the GGT activity present. One unit of GGT is the amount of enzyme that will generate 1.0 μmole of AMC per minute at 37 °C.

SAFETY INFORMATION

Storage Class Code

10 - Combustible liquids

Flash Point(F)

188.6 °F - closed cup

Flash Point(C)

87 °C - closed cup