Glucose Oxidase Activity Assay Kit ยี่ห้อ Sigma
รหัสสินค้า : MAK097
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PROPERTIES
usage
sufficient for 100 colorimetric or fluorometric tests
detection method
colorimetric
fluorometric
storage temp.
−20°C
DESCRIPTION
General description
Glucose Oxidase (GOx, EC 1.1.3.4) is an enzyme found in insects, fungi, and bacteria that catalyzes the oxidation of D-glucose to D-gluconolactone. GOx is widely used in the food, beverage, chemical, and pharmaceutical industries.[1]
Suitability
Suitable for the measurement of glucose oxidase (GOx) in a variety of samples including cells and serum
Principle
The Glucose Oxidase Activity Assay Kit provides a simple and direct procedure for measuring GOx activity in a variety of biological samples. GOx activity is determined by a coupled enzyme assay, in which GOx oxidizes D-glucose resulting in the production of hydrogen peroxide (H2O2) that reacts with a probe, generating a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the GOx present. One unit of GOx is defined as the amount of enzyme that generates 1.0 μmole of H2O2 per minute at 37 °C.
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