PEP Colorimetric/Fluorometric Assay Kit, ยี่ห้อ Sigma

PROPERTIES

usage

sufficient for 100 colorimetric or fluorometric tests

detection method

colorimetric

fluorometric

storage temp.

−20°C

DESCRIPTION

General description

Phospho(enol)pyruvate (PEP) in an intermediate in carbohydrate metabolism involved in glycolysis and gluconeogeneis. In glycolysis, PEP is metabolized by pyruvate kinase to yield pyruvate. In plants, PEP is involved in the formation of aromatic amino acids as well as in the carbon fixation pathway.

Application

PEP Colorimetric/Fluorometric Assay Kit has been used to measure the concentrations of phosphoenolpyruvate.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the measurement of phosphoenolpyruvate (PEP) in tissue, cells and plant extracts

Principle

PEP concentration is determined by a coupled enzyme assay, in which PEP is converted to ATP and pyruvate, resulting in a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the PEP present.