Pgm colorimetric assay kit ยี่ห้อ Sigma

PROPERTIES

usage

sufficient for 100 colorimetric tests

detection method

colorimetric

storage temp.

−20°C

DESCRIPTION

General description

Phosphoglucomutase (PGM) is an enzyme crucial for the interconversion of glucose-1-phosphate and glucose-6-phosphate.[1] In glycogenolysis, PGM converts glucose-1-phosphate into glucose-6-phosphate for use in either the glycolytic pathway or pentose phosphate pathway.[2] When blood glucose levels are high, PGM will convert excess glucose-6-phosphate (formed from glucose by the action of hexokinase) to glucose-1-phosphate.[3]

Deficiency of phosphoglucomutase leads to glycogen storage disease.[4]

Suitability

Suitable for the measurement of phosphoglucomutase activity, analysis of glucose metabolism and cell signaling and screening anti-diabetic drugs in animal tissues (muscle, liver, heart, kidney etc.), cell culture (adherent or suspension cells) and plasma.

Principle

The Phosphoglucomutase Colorimetric Assay kit provides a simple and direct procedure for measuring PGM activity in a variety of samples. PGM activity is determined by a coupled enzyme assay in which glucose-1-phosphate is converted by PGM to glucose-6-phosphate. Glucose-6-phosphate is subsequently oxidized to form a product, which reacts with a probe generating a colorimetric (450 nm) product proportional to the PGM activity present. One unit of PGM is the amount of enzyme that will generate 1.0 μmole of NADH per minute at pH 8.0 at room temperature.