Mtp activity assay kit ยี่ห้อ Sigma

รหัสสินค้า : MAK110

PROPERTIES

usage

sufficient for 100 fluorometric tests

detection method

fluorometric

storage temp.

2-8°C

Gene Information

human ... MTTP(4547)

DESCRIPTION

General description

Microsomal triglyceride transfer protein (MTP), a membrane-bound protein present in the liver, plays an important role in the assembly and secretion of very low density lipoprotein (VLDL).[1] MTP is also necessary for assembly and secretion of chylomicrons by the intestine.[1] This protein facilitates the transfer of triglyceride (TG), cholesteryl ester (CE) and phosphatidylcholine (PC) between phospholipid surfaces. Mutation in the MTP gene leads to abetalipoproteinemia.[1][2]

Application

MTP Activity Assay Kit has been used to measure the activity of microsomal triglyceride transfer protein (MTP).[3]

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for measuring microsomal [triglyceride] transfer protein (MTP) activity in cell lysates or tissue homogenates, or for screening MTP activity inhibitors.

Principle

The MTP Activity Assay Kit is a sensitive, homogeneous fluorometric assay useful for continuous measurement of MTP activity in cell lysate or tissue homogenate. This kit includes proprietary substrates that enable the detection of MTP-mediated transfer of neutral lipid. The transfer activity results in an increase in fluorescence intensity (λEx=465 nm/λEm=535 nm).