Urease Assay Kit ยี่ห้อ Sigma

PROPERTIES

usage

sufficient for 100 colorimetric tests

detection method

colorimetric

storage temp.

2-8°C

DESCRIPTION

General description

Urease is an enzyme that catalyzes the hydrolysis of urea into carbon dioxide and ammonia.[1] Urease is found in bacteria, yeast, and certain higher plant species.[2] Urease activity is found in environmental and fecal samples.[3]

Application

Urease Activity Assay Kit has been used to measure urease activity.[4]

Features and Benefits

Compatible with high-throughput handling systems. Can be adapted for use with cuvettes.

Suitability

Suitable for the detection of Urease activity in biological and environmental samples.

Principle

In this assay, urease catalyzes the hydrolysis of urea resulting in the production of ammonia. The ammonia is determined by the Berthelot method resulting in colorimetric product measure at 670 nm, proportionate to the urease activity present in the sample. One unit of urease is the amount of enzyme that catalyzes the formation of 1.0 μmole ammonia per minute at pH 7.0.