β-Glucosidase Assay Kit ยี่ห้อ Sigma

PROPERTIES

usage

sufficient for 100 colorimetric tests

detection method

colorimetric

storage temp.

−20°C

Gene Information

human ... GBA(2629) , GBA2(57704) , GBA3(57733)

mouse ... GBA(14466) , GBA2(230101)

rat ... GBA(684536) , GBA2(298399) , GBA3(289687)

DESCRIPTION

General description

β-Glucosidase hydrolyzes carbohydrates by acting on terminal, non-reducing b(1→4)-linked D-glucose residues with the release of D-glucose. β-Glucosidases are required by organisms for the consumption of cellulose. Lysozyme, a β-glucosidase present in tears, acts on the β(1→4) glucose bonds present in the peptidoglycan cell wall of Gram-negative bacteria and helps to prevent bacterial infections in the eye. Defects in β-glucosidase activity have been implicated in Gaucher′s disease and Parkinson′s disease.

Application

β-Glucosidase Activity Assay Kit has been used to measure the β-glucosidase activity of the sample.[1]

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

The β-Glucosidase Activity Assay kit provides a simple and direct procedure for measuring β-glucosidase activity in biological samples, for characterizing β-glucosidase production, and for the screening of β-glucosidase modulators.

Principle

In this assay, β-glucosidase activity is determined by a reaction in which β-glucosidase hydrolyzes p-nitrophenyl-β-D-glucopyranoside resulting in the formation of a colorimetric (405 nm) product, proportional to the β-glucosidase activity present. One unit of β-Glucosidse is the amount of enzyme that catalyzes the hydrolysis of 1.0 μmole substrate per minute at pH 7.0.

SAFETY INFORMATION

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2