Monoamine Oxidase Assay Kit ยี่ห้อ Sigma

PROPERTIES

usage

sufficient for 100 fluorometric tests

detection method

fluorometric

storage temp.

−20°C

Gene Information

human ... MAOA(4128) , MAOB(4129)

mouse ... MAOA(17161) , MAOB(109731)

rat ... MAOA(29253) , MAOB(25750)

DESCRIPTION

General description

Monoamine oxidases (MAO, EC 1.4.3.4) are a family of mitochondrial enzymes that catalyze the oxidative deamination of monoamines. In mammals, MAO consists of two isoenzymes, MAO-A and MAO-B. MAO dysfunction is thought to be responsible for a number of neurological disorders. Unusually high or low levels of MAO in the body have been associated with depression, schizophrenia, substance abuse, attention deficit disorder, migraines, and irregular sexual maturation. MAO inhibitors are one of the major classes of drug prescribed for the treatment of depression.

Application

Monoamine Oxidase Activity Assay Kit has been used to estimate MAO-A (monoamine oxidase A)[1][2], MAO-B (monoamine oxidase B)[1] and ALDH (aldehyde dehydrogenase) activities.[2]

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the determination of MAO activity in biological samples and for the screening of MAO inhibitors. Can determine either MAO-A, MAO-B or both MAO-A and MAO-B activity.

Principle

The Monoamine Oxidase Assay Kit provides a convenient fluorimetric means to measure MAO enzyme activity in biological samples. In the assay, MAO reacts with p-tyramine, a substrate for both MAO-A and MAO-B, resulting in the formation of H2O2, which is determined by a fluorimetric method (λex = 530/λem = 585 nm). The assay is simple, sensitive, stable, and high-throughput adaptable. Unit definition: one unit of MAO catalyzes the formation of 1 μmole of H2O2 per minute under the assay conditions.