Phospholipase D Activity Assay Kit ยี่ห้อ Sigma

PROPERTIES

usage

sufficient for 100 colorimetric or fluorometric tests

detection method

colorimetric

fluorometric

storage temp.

−20°C

Gene Information

human ... PLD1(5337) , PLD2(5338) , PLD3(23646) , PLD4(122618) , PLD5(200150) , PLD6(201164)

mouse ... PLD1(18805) , PLD2(18806) , PLD3(18807) , PLD4(104759) , PLD5(319455) , PLD6(194908)

rat ... PLD1(25096) , PLD2(25097) , PLD3(361527) , PLD4(362792) , PLD5(289270) , PLD6(287366)

DESCRIPTION

General description

Phospholipase D (PLD) catalyzes the hydrolysis of the phosphodiester bond of glycerophospholipids to generate phosphatidic acid and choline. It is ubiquitously expressed and is implicated in immune function and cancer inhibition.[1] Abnormalities in PLD expression have been associated with human cancers such as breast, renal, gastric and colorectal cancer.[2]

Application

Phospholipase D (PLD) Activity Assay Kit has been used to measure PLD activity in the liver and lysosomal fractions of the mice liver.[3]

Suitable for the detection of phospholipase D activity in biological samples. This kit can be used to study the effects of various compounds on phospholipase D activity.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the quantitative determination of phospholipase D activity and evaluation of drug effects on phospholipase D metabolism.

Principle

In this assay, PLD hydrolyzes phosphatidylcholine to choline, which is determined using choline oxidase resulting in a colorimetric (570 nm)/fluorometric (λex = 530/λem = 585 nm) product, proportional to the PLD activity in the sample. Unit definition: one unit of PLD catalyzes the formation of 1 mmole choline per minute under the assay conditions (pH 7.4).