Pyrophosphate Assay Kit ยี่ห้อ Sigma
รหัสสินค้า : MAK168
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PROPERTIES
usage
sufficient for 200 fluorometric tests (Blue fluorescence)
detection method
fluorometric
storage temp.
−20°C
DESCRIPTION
General description
Pyrophosphate (PPi) is produced by a number of biochemical reactions such as ATP hydrolysis,[1] DNA and RNA polymerizations, cyclic AMP formation, and the formation of fatty acid-coenzyme A esters. It is hydrolyzed by inorganic pyrophosphatase. PPi is present in the blood and in the extracellular matrix of tissues.[1]
Suitability
The Pyrophosphate Assay Kit provides a simple and direct microplate assay procedure for measuring pyrophosphate in a variety of samples.
Principle
The pyrophosphate concentration of a sample is determined by the use of a unique fluorogenic pyrophosphate sensor in which the presence of pyrophosphate results in the production of a fluorescent product (λex =316/λem = 456 nm) proportional to the pyrophosphate present. This assay is simpler and more robust than traditional enzyme-based methods and is ideal for screening enzyme activity or enzyme inhibitors.
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