Oxalic Acid Colorimetric Assay Kit ยี่ห้อ Sigma

PROPERTIES

detection method

colorimetric

storage temp.

−20°C

DESCRIPTION

General description

Oxalic acid (Oxalate) is a strong dicarboxylic acid[1] that occurs in several plants and vegetables. In animals, it is either incorporated from the diet or produced during ascorbic acid[2] and glyoxylic acid metabolism. Oxalic acid is not metabolized and is excreted in the urine. Hence, it can act as a marker for glyoxylic and ascorbic acid metabolism. Furthermore, oxalic acid has been studied as a pathogenic factor for Sclerotinia sclerotiorum that inhibits the oxidative burst response in host plants.[3] It is also known to alleviate chilling injury in peach fruit. Elevated levels of oxalic acid (oxalate) have been associated with the formation of kidney stones.[4] Hence, oxalate measurements can be used for the diagnosis and monitoring of renal stones.

Application

Oxalic acid colorimetric assay kit has been used in 24-hour urinary oxalate level (24HUOL) evaluation in an animal model.[5]

Suitability

Suitable for the detection of oxalate (oxalic acid) in biological samples including animal and plant tissue.

Principle

The Oxalic Acid Assay Kit provides a simple and sensitive procedure for measuring oxalic acid in variety of samples including animal and plant tissue. In this assay, oxalic acid concentration is determined by a coupled enzyme reaction, which results in a colorimetric (450 nm) product, proportional to the oxalate present.