Pdh activity assay kit ยี่ห้อ Sigma

PROPERTIES

usage

sufficient for 100 colorimetric tests

detection method

colorimetric

storage temp.

−20°C

Gene Information

human ... PDHA1(5160) , PDHA2(5161) , PDHB(5162) , PDHX(8050)

mouse ... PDHA1(18597) , PDHA2(18598) , PDHB(68263) , PDHX(27402)

rat ... PDHA1(29554) , PDHA2(117098) , PDHB(289950) , PDHX(311254)

DESCRIPTION

General description

Pyruvate dehydrogenase (PDH) is a mitochondrial enzyme that catalyzes the conversion of pyruvate to acetyl-CoA and CO2, and also links the tricarboxylic acid (TCA) and glycolysis pathways.[1] The enzyme is inhibited by phosphorylation and activated by dephosphorylation. Mutations in PDH have been linked to pyruvate dehydrogenase deficiency (causing lactic acidosis and neurologic dysfunctions) and Leigh syndrome.[2] PDH has also been implicated in oncogene-induced senescence.[3] PDH measurements can provide insights into metabolic functions and oncogenesis.

Application

PDH Activity Assay Kit has been used to determine the activity of pyruvate dehydrogenase.[4][5][6]

Suitability

Suitable for the detection of PDH activity in a variety of biological samples such as cell and tissue culture supernatants and purified mitochondria.

Principle

Pyruvate dehydrogenase activity is determined using a coupled enzyme reaction, which results in a colorimetric (450 nm) product proportional to the enzymatic activity present. One unit of pyruvate dehydrogenase is the amount of enzyme that will generate 1.0 mmole of NADH per minute at 37 °C.