Succinate colorimetric assay kit ยี่ห้อ Sigma

PROPERTIES

detection method

colorimetric

storage temp.

−20°C

DESCRIPTION

General description

Succinate (succinic acid) is a citric acid cycle intermediate and functions in the transport of electrons to the mitochondrial electron transport chain.[1][2] It also functions in innate immune signaling and induces IL-1β production during inflammation. Succinates have been often used for the treatment of hypertension.[3] Succinate measurements can be useful for studying its metabolic, immunological, and therapeutic activities.

Application

Succinate Colorimetric Assay Kit has been used to measure the concentration of succinate.[4][5]

Suitability

Suitable for the detection of succinate in a variety of samples such as cell and tissue culture supernatants and in liquid samples such as wine.

Principle

The Succinate Colorimetric Assay Kit is a highly sensitive assay for determining succinate levels (ranging from 2-10 nmole/well) in a variety of samples. Succinate concentration is determined by a coupled enzyme reaction, which results in a colorimetric (450 nm) product proportional to the succinate present.