Urokinase activity flurometric assay kit ยี่ห้อ Sigma

PROPERTIES

detection method

fluorometric

storage temp.

−20°C

Gene Information

human ... PLAUH(5328)

mouse ... PLAUH(18792)

rat ... PLAUH(25619)

DESCRIPTION

General description

Urokinase (Urokinase-type plasminogen activator, uPA) is a serine protease that catalyzes the conversion of plasminogen to active plasmin. Urokinase is involved in diverse cellular processes including blood coagulation, cell adhesion, fibrinolysis, and cell migration. Studies in cultured fibroblasts have revealed urokinase induces MMP-9 expression and reactive oxygen species (ROS) generation. Suppression of uPA expression has been studied for therapeutic implications in hepatocellular carcinoma (HCC). Urokinase activity measurements may be useful for the study of cell functions and cancer therapy.

Suitability

Suitable for the detection of Urokinase Activity in variety of samples such as serum, cell and nuclear extracts, and with purified enzymes.

Principle

The Urokinase Activity Fluorometric Assay Kit provides a simple and direct procedure for measuring urokinase activity (ranging from 0.01-0.5 IU/well) in a variety of samples. Urokinase activity is determined using the enzymatic cleavage of an AMC-based (amido-methylcoumarin) peptide substrate, which results in the generation of AMC (λex = 350/λem = 450 nm) proportional to the enzymatic activity present.